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目的观察依硫磷酸对人慢性髓细胞白血病K562细胞系增殖的抑制作用。方法将依硫磷酸(粉末)以1640培养液稀释成0.2mg/ml、0.5mg/ml、0.75mg/ml、1.0mg/ml、1.6mg/ml、3.2mg/ml共6个浓度组,分别处理增殖期人慢性髓细胞白血病K562细胞,镜下观察不同时间点各浓度组细胞生长情况,应用CCK-8法检测细胞增殖能力,绘制生长曲线,计算抑制率和半数抑制浓度(IC50)。结果依硫磷酸对K562细胞增殖具有抑制作用,细胞倍增时间为48h,并且具有时间和剂量依赖性。在给药48h>0.75mg/ml浓度的依硫磷酸对K562细胞增殖具有抑制作用(P<0.05),而3.2mg/ml浓度的依硫磷酸与K562细胞培养72h后对其增殖抑制作用最强,抑制率为91%。结论依硫磷酸对K562细胞增殖具有抑制作用,为临床应用依硫磷酸治疗慢性髓细胞白血病提供了实验基础。
Objective To observe the inhibitory effect of sodium thiosulfate on the proliferation of human chronic myelogenous leukemia K562 cell line. Methods Thiophosphate (powder) was diluted to a concentration of 0.2mg / ml, 0.5mg / ml, 0.75mg / ml, 1.0mg / ml, 1.6mg / ml and 3.2mg / The proliferation of human chronic myeloid leukemia K562 cells was observed. The growth of cells in different concentrations at different time points was observed. Cell proliferation was measured by CCK-8 assay, and the growth curve was calculated. The inhibition rate and half-maximal inhibitory concentration (IC50) were calculated. RESULTS: Methionine had inhibitory effect on the proliferation of K562 cells. The cell doubling time was 48h, and it was time-and dose-dependent. (P <0.05), and the inhibitory effect on the proliferation of K562 cells was inhibited at the dose of> 0.75mg / ml 48h (P <0.05), while the inhibitory effect on the proliferation of K562 cells was highest at the concentration of 3.2mg / ml , The inhibition rate was 91%. CONCLUSION: Imethionine can inhibit the proliferation of K562 cells and provide the experimental basis for clinical treatment of chronic myeloid leukemia with thiophosphate.