论文部分内容阅读
应用光敏生物素标记细粒棘球绦虫特异性DNA探针pEG18,对新疆5个不同地区绵羊、1例肝包虫病人、阿勒泰地区牛细粒棘球绦虫原头节以及羊源、牛源细粒棘球绦虫成虫DNA进行限制性酶切谱和Southern杂交分析。牛源、羊源样本经EcoRI酶切后产生的杂交图型基本上是一致的。BamHI酶切后产生的杂交结果,在新疆5个不同地区绵羊感染的细粒棘球绦虫没有发现差别,而人源和牛源细粒棘球绦虫DNA产生的杂交图型与绵羊源细粒棘球绦虫DNA产生的杂交图型大体上是一致的,但又存在着各自的差异。与绵羊样本相比,人源细粒棘球绦虫产生的杂交图型约在8.3kb处明显可见一条杂交带,而牛源细粒棘球绦虫约在3.5kb处有一条清晰的杂交带。这些差异的意义有待进一步研究。用光敏生物素代替同位素标记DNA探针,简便易行,稳定可靠,可取得满意的结果,在边远的地区是一种值得推广的方法。
Photobiotin-labeled Echinococcus granulosus-specific DNA probe pEG18 was used to detect the expression of Echinococcus granulosus in five different regions in Xinjiang, one case of hepatic hydatid disease, the original head of Echinococcus granulosus in Altay, Restriction enzyme digestion and Southern hybridization analysis of DNA from adult Echinococcus granulosus. Cattle source, sheep samples by EcoRI digestion after the hybrid pattern is basically the same. BamHI digestion results produced hybridization in Xinjiang 5 different regions of sheep infected with Echinococcus granulosus did not find any difference between human and bovine source DNA of Echinococcus granulosus hybrid map and sheep origin Echinacea The hybrid patterns produced by tapeworm DNA are generally identical, but there are also differences between them. Compared with the sheep sample, the hybridization pattern produced by human Echinococcus granulosus shows a hybrid band at about 8.3 kb, whereas the Euglena granulosus has a clear hybrid band at about 3.5 kb. The significance of these differences needs further study. Replacing isotope labeled DNA probes with photobiotin is simple, reliable, and achieves satisfactory results. It is worth promoting in remote areas.