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目的:观察维生素E对实验性慢性胰腺炎的生化和病理形态影响。方法:SD大鼠共分6组,即模型组、维生素E组和各种对照组(乙醇对照组,含10%乙醇PBS胰管内注射;NS对照组,等体积NS胰管内注射;假手术组,假手术,胰管内没有注射;正常对照组,没有手术的正常大鼠);维生素E组每日400 mg/kg体重灌胃,其余各组等体积生理盐水灌胃,治疗4周。处死大鼠后检测血浆维生素E和过氧化脂质水平、HE和胶原纤维染色观察胰腺病理改变。结果:血浆维生素E浓度在维生素E组明显高于模型组和各对照组(P<0.05),模型组与各对照组的差异没有统计学意义(P>0.05)。血浆过氧化脂质在维生素E组明显低于模型组和各对照组(P<0.05),而各对照组又明显低于模型组(P<0.05)。模型组大鼠胰腺腺泡细胞坏死、局灶萎缩、小叶内和小叶间广泛纤维化、伴炎症细胞浸润,符合人类慢性胰腺炎的病理特征。大鼠胰腺纤维化、腺泡萎缩和炎症细胞浸润在维生素E组明显减轻(P<0.05)。结论:维生素E可以有效改善慢性胰腺炎大鼠的病理形态,缓解胰腺纤维化程度,提高血维生素E水平,降低过氧化脂质。
Objective: To observe the effects of vitamin E on the biochemical and pathological features of experimental chronic pancreatitis. Methods: SD rats were divided into 6 groups: model group, vitamin E group and various control groups (ethanol control group, 10% ethanol in PBS; pancreatic duct in NS control group, , Sham operation, there was no injection in the pancreatic duct; normal control group, normal rats without surgery); the vitamin E group was given intragastrically 400 mg / kg body weight daily, and the other groups were given normal saline for 4 weeks. The rats were killed after the test of plasma vitamin E and lipid peroxidation levels, HE and collagen fibers were stained to observe the pathological changes of the pancreas. Results: The plasma vitamin E concentration in vitamin E group was significantly higher than that in model group and each control group (P <0.05). There was no significant difference between model group and each control group (P> 0.05). Plasma lipid peroxidation in vitamin E group was significantly lower than that in model group and each control group (P <0.05), while that in control group was significantly lower than that in model group (P <0.05). Pancreatic acinar necrosis, focal atrophy, extensive fibrosis in the interlobular and interlobular lobes, and infiltration of inflammatory cells in the model group were consistent with the pathological features of human chronic pancreatitis. Rats with pancreatic fibrosis, acinar atrophy and inflammatory cell infiltration in vitamin E group was significantly reduced (P <0.05). Conclusion: Vitamin E can effectively improve the pathological changes of rats with chronic pancreatitis, relieve the degree of pancreatic fibrosis, raise the level of serum vitamin E and decrease the lipid peroxidation.