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基于超声波辅助提取藜麦中碳苷黄酮类化合物的工艺研究,建立了反相高效液相色谱法同时检测荭草素、异荭草素和牡荆素的方法。藜麦种子打粉过筛后,以90%的甲醇为提取溶剂,采用3因素3水平正交试验考察,得出超声波提取的最佳工艺条件为:超声功率80 W,超声时间30 min,超声温度55℃;在Diamonsil-C_(18)分离柱,酸性甲醇-水流动相体系,合适梯度洗脱程序下以恒流速1.0 mL/min,35℃柱温条件分离测定藜麦样品中的荭草素、异荭草素和牡荆素;结果显示,3种碳苷黄酮类物质分别在5.6~90μg/mL、2.66~42.5μg/mL和3.28~52.5μg/mL的范围内线性关系良好,定量限分别为0.519,0.416和0.322μg/mL,精密度和重复性的RSD分别为0.76%~1.63%、0.57%~1.78%,加样平均回收率为93.73%~101.68%,RSD为0.51%~2.55%;该方法精密度、重复性和稳定性良好,可用于藜麦样品中3种碳苷黄酮的分离检测。
Based on the ultrasonic-assisted extraction of carbon glycoside flavonoids in quinoa, a method was developed for the simultaneous determination of tenardin, isoorientin and vitexin by RP-HPLC. After the quinoa seeds were powdered and sieved, 90% methanol was used as the extraction solvent. The optimal conditions of ultrasonic extraction were as follows: ultrasonic power 80 W, ultrasonic time 30 min, ultrasonic temperature 55 ℃. The quantification of toxin in the quinoa sample was carried out on a Diamonsil-C_ (18) column and an acidic methanol-water mobile phase at a constant flow rate of 1.0 mL / min at 35 ℃ under a suitable gradient elution program. , Isohumulone and vitexin. The results showed that the three kinds of carbohydrate glycosides showed good linearity in the range of 5.6 ~ 90μg / mL, 2.66 ~ 42.5μg / mL and 3.28 ~ 52.5μg / mL respectively. The limit of quantification RSD of precision and repeatability were 0.76% ~ 1.63% and 0.57% ~ 1.78%, respectively. The average recoveries were 93.73% ~ 101.68% and RSD were 0.51% ~ 2.55 %. The method has good precision, repeatability and stability and can be used for the separation and detection of three kinds of carbohydrate flavonoids in quinoa samples.