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目的为椰毒假单胞菌酵米面亚种食物中毒的实验室分离鉴定寻找更快速、准确的方法。方法采用增菌和不增菌2种方法,比较菌落生长情况;PDA平板添加或不添加抗生素,比较平板分离效果。结果不论增菌还是不增菌,PDA平板目标菌的数量无差异,经过增菌的平板杂菌比不增菌的数量多;添加与不添加抗生素的平板目标菌生长速度无差异,添加抗生素的平板菌落比不添加的略大,椰酵假单胞菌生长更纯。结论在椰毒假单胞菌酵米面亚种的鉴定过程中,同时开展增菌和不增菌培养,既可节约时间、成本,又避免漏检,提高了检出率;PDA平板添加2种抗生素,使培养基选择性更强,菌落特征显著。
Objective To find a faster and more accurate method for laboratory isolation and identification of Pseudomonas solanacearum subsp. Methods Two methods of adding bacteria and not adding bacteria were used to compare the growth of colony. PDA plate was added with or without antibiotics to compare the effect of plate separation. The results showed that there was no difference in the number of target bacteria in PDA plates, no increase in the number of target bacteria in PDA plates, and no increase in the number of target bacteria in plate-bearing bacteria with or without antibiotics. Plate colonies were slightly larger than those not added, and P. aeruginosa grew more purely. Conclusions During the identification process of S. coco Pseudoalterium, the simultaneous and un-enrichment culture can save time and cost, avoid missed inspection and increase the detection rate. PDA plate added two kinds Antibiotics make the medium more selective and have significant colony characteristics.