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目的:建立广西产金樱子的HPLC指纹图谱,并进行聚类分析和主成分分析,为评价其质量提供依据。方法:采用十八烷基硅烷键合硅胶为固定相,以甲醇-0.2%磷酸水溶液作为流动相,进行梯度洗脱,柱温25℃,流速0.8 m L·min-1,检测波长255 nm,进样量10μL。采用中药色谱指纹图谱相似度评价系统2012.1版软件对10批样品进行共有峰确认及相似度评价。运用SPSS 22.0统计学软件对HPLC指纹图谱进行聚类分析和主成分分析。结果:色谱评价软件共标定出共有色谱峰13个,从中选择了10个稳定性好,吸收峰强,特征明显的色谱峰作为金樱子指纹图谱的共有峰。用对照品指认了其中的3个化学成分,分别为儿茶素、橙皮苷、芦丁。10批金樱子样品指纹图谱的相似度均>0.96。通过聚类分析,将不同产地的10批样品分为3类,并且2个主成分的累计方差贡献率为82.98%。结论:该研究能为广西金樱子质量标准的提高提供数据支持,并为其品质与开发利用提供评价依据。
OBJECTIVE: To establish HPLC fingerprinting of Rosa laevigata in Guangxi and carry out cluster analysis and principal component analysis to provide the basis for evaluating its quality. Methods: The octadecylsilane bonded silica gel was used as the stationary phase. The mobile phase consisted of methanol-0.2% phosphoric acid solution with gradient elution. The column temperature was 25 ℃ and the flow rate was 0.8 m L · min-1. The detection wavelength was 255 nm, Injection volume 10μL. Using Chinese medicine chromatographic fingerprint similarity evaluation system 2012.1 version of the software to 10 batches of samples for the common peak identification and similarity evaluation. SPSS 22.0 statistical software was used to carry out cluster analysis and principal component analysis on HPLC fingerprints. Results: A total of 13 common chromatographic peaks were calibrated by the chromatographic evaluation software. Ten peaks with good stability, strong absorption and obvious characteristics were selected as the common peaks of the fingerprints of Rosa laevigata. With the reference substance which identified three of the chemical composition, namely catechin, hesperidin, rutin. The fingerprints of 10 samples of Rosa laevigata showed similarity of> 0.96. By cluster analysis, 10 batches of samples from different producing areas were divided into three categories, and the cumulative variance of the two principal components was 82.98%. Conclusion: This study can provide data support for the improvement of quality of Rosa laevigata in Guangxi, and provide the basis for its quality and development and utilization.