论文部分内容阅读
【目的】大葱在贮藏期频繁发生镰孢菌腐烂病,损失严重。明确该病害病原种类对病害防治具有重要意义。【方法】利用组织分离法对采集自甘肃省兰州市(区)蔬菜市场的16份大葱贮藏期镰孢菌腐烂病病样进行病原物的分离、纯化培养,经单孢分离后根据形态学特征,再结合r DNA-ITS、EF-1a(tef)基因序列分析的方法进行鉴定。【结果】共分离得到80株镰孢菌,经鉴定分属3个种,即层出镰孢菌(Fusarium proliferatum)、尖孢镰孢菌(F.oxysporum)和燕麦镰孢菌(F.avenaceum),其中层出镰孢菌为大葱镰孢菌腐烂病的优势致病菌,分离频率为52.50%。对兰州白葱不同部位进行致病性测定,结果表明层出镰孢菌对大葱鳞茎的致病力最强,而燕麦镰孢菌对大葱鳞茎的致病力最弱。【结论】3种镰孢菌作为该病害的病原,属国内首次报道。
【Objective】 Onion frequently suffered from Fusarium rot in the storage period, with serious loss. Clear pathogen species of disease prevention and treatment of great significance. 【Method】 Tissue separation method was used to isolate, purify and culture 16 Fusarium venenatum samples collected from the vegetable market of Lanzhou (Gansu Province) in Gansu Province during the storage period. According to the morphological characteristics , And then combined with rDNA-ITS, EF-1a (tef) gene sequence analysis method for identification. 【Result】 A total of 80 Fusarium isolates were isolated and identified as belonging to three species, namely Fusarium proliferatum, F. oxysporum and F. avenae ), In which Fusarium venenatum was the dominant pathogen of Fusarium venenatum rot. The frequency of separation was 52.50%. The pathogenicity test of different parts of Lanzhou white scallion showed that the pathogenicity of Fusarium graminearum to Bulbophyllum was the strongest, while the virulence of Fusarium oxysporum to scallion bulb was weakest. 【Conclusion】 The three Fusarium pathogens were the first reported in China.