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吖叮橙染色法萤光显微镜检术对诊断胃癌的临床应用。赵实邦等(山东医学院附属医院内科教研组)。中华医学杂志51:311,1965。 5例胃肿瘤部涂片用Schiffer氏法在纯甲醇和Carnoy氏液中顺序固定3分钟,于后80％乙醇浸5秒,70％乙醇5秒,50％乙醇5分,蒸馏水轻盪10秒,1％醋酸5秒,蒸馏水2分钟,再轻盪10秒,缓冲液5分钟,0.01％吖叮橙浸冲液2分钟,缓冲液3分钟,再轻盪4分钟,缓冲液4分钟。镜检时加Mellvan氏缓冲液(pH6.4)1滴封盖。5例癌细胞为圆形或椭圆形,胞浆呈桔红色,萤光均匀。胞核较大,约占细胞的l/2—2/3,呈黄白色萤光,核内含有1—2个桔红色核
Clinical application of fluorescein microscopy with cesium orange staining for diagnosis of gastric cancer. Zhao Shibang et al. (Institute of Internal Medicine, Affiliated Hospital of Shandong Medical College). Chinese Medical Journal 51:311,1965. Five cases of gastric tumors were smeared with Schiffer’s method in pure methanol and Carnoy’s solution for 3 minutes, followed by 80% ethanol for 5 seconds, 70% ethanol for 5 seconds, 50% ethanol for 5 minutes, and distilled water for 10 seconds. 1% acetic acid for 5 seconds, distilled water for 2 minutes, followed by 10 seconds of gentle shaking, 5 minutes for the buffer, 0.01% dip in the orange for 2 minutes, 3 minutes for the buffer, 4 minutes for the light, 4 minutes for the buffer. Add 1 drop of Mellvan’s buffer (pH 6.4) to the lens for microscopic examination. In 5 cases, the cancer cells were round or oval, and the cytoplasm was orange-red and evenly fluorescent. The nucleus is relatively large, accounting for about l/2-2/3 of the cells, showing yellow-white fluorescence, and the nucleus contains 1-2 orange-colored nuclei.