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用ＭＴＴ比色分析法和流式细胞术在体外研究了５肽胃泌素（ｐｅｎｔａｇａｓｔｒｉｎ，ＰＧ）及胃泌素受体拮抗剂丙谷胺（ｐｒｏｇｌｕｍｉｄｅ，ＰＧＬ）对人大肠癌细胞株ＳＷ４８０的作用。结果显示：ＰＧ能促进ＳＷ４８０细胞生长；ＰＧＬ单用时对ＳＷ４８０细胞没有影响，当ＰＧ与ＰＧＬ合用时，ＰＧＬ呈剂量依赖地拮抗ＰＧ的作用，ＰＧＬ在３２μｇ／ｍｌ以上时，拮抗作用趋于恒定。提示胃泌素的作用是通过其受体发挥的。ＰＧ促进ＳＷ４８０细胞合成ＤＮＡ、蛋白质，促进ＳＷ４８０细胞从Ｇ０／Ｇ１期过度到Ｓ期和Ｇ２Ｍ期；ＰＧＬ完全拮抗这种作用，提示胃泌素的作用在受体后是通过影响细胞周期实现的
The effects of pentagastrin (PG) and gastrin receptor antagonist proglumide (PGL) on human colorectal cancer cell line SW480 were studied in vitro by MTT colorimetric assay and flow cytometry. . The results showed that PG could promote the growth of SW480 cells; PGL alone had no effect on SW480 cells. When PG was combined with PGL, PGL antagonized the effect of PG in a dose-dependent manner. When PGL was above 32 μg/ml, the antagonism tended to be constant. It is suggested that the effect of gastrin is exerted through its receptor. PG promotes the synthesis of DNA and protein in SW480 cells and promotes the transition from G0/G1 phase to S phase and G2M phase in SW480 cells; PGL completely antagonizes this effect, suggesting that the effect of gastrin on the receptor is through affecting the cell cycle.