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为明确引起樱桃褐斑病的病原菌种类,采集泰安、潍坊、烟台3个地区典型发病叶片,用单孢分离法分离、纯化,共获得16个菌株,采用病原菌形态学与分子生物学相结合的方法,鉴定樱桃褐斑病的病原菌。对该病原菌的ITS序列分析显示,其与Gen Bank中登录的Passalora sp.属的Passalora arctostaphyli相似性达97%;与另一种在甜樱桃叶片中分离的病原菌Pseudocercospora pruni-persicicola相似度仅为85.6%,证明两者并非为同一属真菌。构建系统进化树表明,樱桃褐斑病病原与Passalora sp.属真菌处在同一分支,而与其它Prunus sp.属分离的病原菌处在两个分支,进而通过形态学鉴定和ITS序列分析认为,病原菌为Passalora circumscissa,并将其ITS基因序列提交至Gen Bank数据库(基因登陆号:KT428056)。
In order to clarify the pathogen species causing cherry leaf spot, we collected three typical leaves from Tai’an, Weifang and Yantai and isolated and purified them by single spore separation method. Sixteen strains were obtained. The morphological and molecular biology of pathogen Methods to identify cherry brown spot pathogens. ITS sequence analysis of this pathogen revealed 97% similarity to Passalora arctostaphyli in Passalora sp. Registered in GenBank and 85.6% similarity to another Pseudocercospora pruni-persicicola isolate in sweet cherry leaves %, Prove that the two are not the same fungi. The phylogenetic tree indicated that the pathogen of cherry leaf spot was on the same branch as Passalora sp. And the pathogen isolated from other Prunus sp. Was on two branches, and then the morphological identification and ITS sequence analysis suggested that the pathogen Is Passalora circumscissa and its ITS gene sequence is submitted to the Gen Bank database (Gene Accession Number: KT428056).