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目的 探讨 p5 3、 bcl- 2基因在人脑星形细胞瘤发生发展中的作用 ,以及上述基因蛋白检测对星形细胞瘤的诊断、分级的临床意义。方法 采用免疫组化 S- P染色法对 47例人脑星形细胞瘤组织蜡块标本进行 p5 3、 bcl- 2基因蛋白检测。结果 47例脑星形细胞瘤中 p5 3、 bcl- 2蛋白的阳性率分别为 48.9% (2 3/ 47)、 5 7.4% (2 7/ 46 ) ,其中多形性胶质母细胞瘤的 p5 3基因蛋白阳性水平高于星行细胞瘤 ,而 bcl- 2蛋白阳性水平则是星行细胞瘤高于多形性胶质母细胞瘤。结论 p5 3、bcl-2基因的异常表达与人脑星形细胞瘤的发生、发展有密切关系 ,对星形细胞瘤的病理分级和预后判断有积极的意义
Objective To investigate the role of p53 and bcl-2 in the development of human astrocytoma and the clinical significance of the detection of the above protein in astrocytomas. Methods Immunohistochemical S-P staining was used to detect p53 and bcl-2 gene in 47 human astrocytoma paraffin blocks. Results The positive rates of p53 and bcl-2 in 47 cases of astrocytomas were 48.9% (23/47) and 57.4% (27/46), respectively. The positive rates of p53 and bcl-2 protein in glioblastoma multiforme The positive level of p5 3 protein was higher than that of astrocytoma, while the positive level of bcl-2 protein was higher in astrocytoma than glioblastoma multiforme. Conclusion The abnormal expression of p53 gene and bcl-2 gene is closely related to the occurrence and development of human astrocytoma and has a positive effect on the pathological grade and prognosis of astrocytomas