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本文证明超螺旋DNA在一定条件下与乙二醛反应后,空间结构会发生明显的变化。利用乙二醛与DNA反应的可逆性,作者用电子显微镜观察到了超螺旋DNA不同形态之间的拓扑变换。根据反应产物的电泳行为和电子显微镜下的形态,很难用DNA的Watson-Crick模型来解释,本文作者提出了双链DNA中同时存在左旋和右旋结构的DNA三级结构的假说,似乎比较好地解释了各种有关事实和现象。
This paper shows that under certain conditions, supercoiled DNA reacted with glyoxal, the spatial structure will change significantly. Using the reversibility of the reaction of glyoxal with DNA, the authors observed the topological transformation between different morphologies of supercoiled DNA using electron microscopy. According to the electrophoretic behavior of the reaction product and the morphology under the electron microscope, it is very difficult to explain the Watson-Crick model of DNA. The author proposed the hypothesis that DNA double-stranded DNA has both levorotatory and dextrorotatory structures in tertiary structure, Well explained the various facts and phenomena.