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为研究载脂蛋白Ｂ（ａｐｏＢ）基因限制性片段长度多态性与动脉粥样硬化性脑梗死（ＡＣＩ）的关系，采用聚合酶链反应（ＰＣＲ）技术检测了国人８５例ＡＣＩ和５６例正常人（ＮＣ）的ａｐｏＢ基因的ＸｂａⅠ位点多态性。结果显示：中国人ＸｂａⅠ位点等位基因频率与国外种族有差异。本研究Ｘ＋等位基因频率高于日本人、新加坡人，低于印度人、南亚人及欧洲人。ＮＣ组Ｘ－Ｘ＋基因型血浆总胆固醇（ＴＣ）水平明显高于Ｘ－Ｘ－基因型（Ｐ＜０．００１）。Ｘ－Ｘ－基因型个体：ＡＣＩ组血浆ＴＣ、甘油三酯（ＴＧ）、ａｐｏＢ、Ｌｐ（ａ）水平明显高于ＮＣ组，ａｐｏＡ水平明显低于ＮＣ组。提示：Ｘ－Ｘ－基因型可能与ＡＣＩ患者血脂异常有关。
To investigate the relationship between the restriction fragment length polymorphism of apolipoprotein B (apoB) gene and atherosclerotic cerebral infarction (ACI), polymerase chain reaction (PCR) was used to detect 85 ACI patients and 56 normal controls Human (NC) apoB gene Xba I site polymorphism. The results showed that there was a difference in allele frequency of Xba Ⅰ locus in Chinese from that in other countries. The frequency of X + allele in this study was higher than that of Japanese, Singaporeans, Indian, South Asian and Europeans. The level of total cholesterol (TC) in X-X + genotype of NC group was significantly higher than that of X-X- genotype (P <0.001). Individuals with X-X-genotype: Plasma TC, triglyceride (TG), apoB and Lp (a) in ACI group were significantly higher than those in NC group and apoA level was significantly lower than NC group. Tip: X-X- genotype may be related to dyslipidemia ACI patients.