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为了观察茶色素(Tea Pigment,TP)对离休豚鼠心肌氧自由基损伤的保护作用。分别将豚鼠右室前乳头肌,置于Tyrode液恒温浴槽中,通O_2(95％)和CO_2(5％)混合气体。实验组加黄嘌呤-黄嘌呤氧化酶系统(X-XOD)产生氧自由基诱发乳头肌氧化损伤;另一组在加X-XOD之前5min先加入TP25mg·L~(-1)。分别观察各组心室乳头肌的生理特性变化,最后测定各标本匀浆中超氧化歧化酶(SOD)和脂质过氧化物丙二醛(MDA)的含量。结果表明TP能显著对抗氧自由基损伤诱导的心肌生理特性的变化,对心肌组织中SOD活性降低及MDA含量升高,有明显对抗作用。由此推论TP对豚鼠离体心肌氧自由基损伤有显著的保护作用。
In order to observe the protective effect of Tea Pigment (TP) on myocardial oxygen free radical injury in the guinea pigs after resignation. The right ventricular papillary muscles of guinea pigs were placed in a constant temperature bath of Tyrode’s solution, and a mixed gas of O 2 (95%) and CO 2 (5%) was used. The xanthine-xanthine oxidase system (X-XOD) produced oxygen free radicals in the experimental group to induce oxidative damage to the papillary muscles; the other group was supplemented with TP 25 mg·L -1 5 min before adding X-XOD. The physiological characteristics of papillary muscles in each group were observed, and the contents of superoxide dismutase (SOD) and lipid peroxide malondialdehyde (MDA) in the homogenates of each specimen were determined. The results showed that TP can significantly counteract the changes in myocardial physiological characteristics induced by oxygen free radical injury, and have a significant antagonistic effect on SOD activity and MDA content in myocardial tissue. It was concluded that TP has a significant protective effect on isolated myocardial oxygen free radical damage in guinea pigs.