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目的:建立替尼泊苷脂质体包封率的测定方法。方法:采用SephadexG-50分离脂质体与游离药物。以500r.min-1离心3min制备微柱,加样量0.1mL,洗脱液为0.9%氯化钠水溶液,洗脱条件为2000r.min-1,离心5min。采用HPLC法测定过柱后脂质体中药物含量和过柱前脂质体中药物含量,通过上述二者之比计算替尼泊苷脂质体的包封率。结果:空白脂质体的柱回收率为98.48%(n=3),药物的柱回收率为98.69%(n=3);洗脱曲线研究结果表明在本实验中脂质体组分与游离药物组分之间有很好的分离度。微型凝胶柱离心法测定自制替尼泊苷脂质体包封率为(80.89±1.92)%。结论:采用SephadexG-50微柱凝胶离心分离脂质体与游离药物,再用HPLC法测定替尼泊苷脂质体中药物包封率,简便快速,结果重复性好。
Objective: To establish a method for determination of entrapment efficiency of teniposide liposomes. Methods: Sephadex G-50 was used to separate liposomes and free drugs. Centrifuge at 500 r.min-1 for 3 min to prepare a microcolumn. The volume of the sample was 0.1 mL, and the eluate was 0.9% sodium chloride aqueous solution. The elution conditions were 2000 r.min-1 and centrifuged for 5 min. HPLC method was used to determine the drug content in the liposomes and the drug content in the liposomes before the column was passed, and the encapsulation efficiency of the teniposide liposomes was calculated by the above ratio. Results: The column recovery of the blank liposomes was 98.48% (n = 3), the column recovery of the drug was 98.69% (n = 3). The results of elution curve showed that in this experiment, There is a good degree of resolution between the drug components. The encapsulation efficiency of self-made teniposide liposomes was (80.89 ± 1.92)% by micro-gel column centrifugation. Conclusion: Separation of liposomes and free drugs by Sephadex G-50 microcolumn gel centrifugation was performed. The entrapment efficiency of liposomal teniposide was determined by HPLC.