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为了寻找与小麦雌性育性紧密关联的SSR分子标记,并估算其表型效应,选择均匀分布于小麦基因的63个SSR标记,对包括小麦雌性不育系XND126在内的261个品种(系)组成的群体进行分析,并使用STRUCTRE软件进行群体结构评估,利用TASSEL软件的GLM模型检测多态性标记与小麦雌性育性(国内法育性D.F.和国际法育性I.F.)两种表型值的关联程度。结果表明:目标群体中存在3个亚群。基因组中多态性标记关联分析初步显示,标记Xcfd36(2AS、2DS)和Xcfd88(4AL)与小麦雌性育性两种表型值相关联,两个标记对D.F.和I.F.的效应分别是0.1585,0.113和0.087,0.0596。进一步分析发现2DS和4AL连锁群上存在一些与小麦雌性育性更加紧密关联的标记,如Xwmc25(2DS)和Xwmc232(4AL),其效应分别可达到0.5833和0.2841,而2AS上未找到独有的关联标记。分析认为小麦染色体2DS和4AL各存在一个控制雌性育性的基因位点,其中2DS上的QTL被连锁分析所证实。对与表型紧密关联的标记的鉴别对于小麦雌性育性QTL发掘的重要作用进行了探讨。
In order to find SSR molecular markers closely related to female fertility and to estimate their phenotypic effects, 63 SSR markers evenly distributed on wheat genes were selected and 261 SSR markers including wheat female sterile line XND126 were selected. The population structure was analyzed and STRUCTRE software was used to evaluate the population structure. The GLASS model of TASSEL software was used to detect the association of polymorphic markers with the phenotypic values of female fertility (domestic fertility DF and international fertility IF) degree. The results show that there are 3 subpopulations in the target population. Correlation analysis of the polymorphism markers in the genome showed that the markers Xcfd36 (2AS, 2DS) and Xcfd88 (4AL) were correlated with the phenotypic values of female fertility in wheat. The effects of the two markers on DF and IF were 0.1585,0.113 And 0.087,0.0596. Further analysis revealed that there were some markers that were more closely related to female fecundity in 2DS and 4AL linkage groups, such as Xwmc25 (2DS) and Xwmc232 (4AL), with effects of 0.5833 and 0.2841, respectively, but no unique Correlation mark. Analysis shows that there is a locus controlling female fertility in wheat chromosomes 2DS and 4AL, among which the QTL on 2DS is confirmed by linkage analysis. The identification of markers closely related to the phenotype has also explored the important role of QTL in female fecundity.