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目的本研究探讨错配修复基因h MLH1、h MSH2及h MSH6的表达与宫颈病变的关系、在宫颈肿瘤形成及发展过程中的作用。方法采用免疫组化SP法检测错配修复基因h MLH1、h MSH2、h MSH6在50例慢性宫颈炎~CINI组,50例CINII~CIN III组,50例宫颈癌组中的表达。结果 h MLH1蛋白的缺失表达率在慢性宫颈炎~CINI组、CINII~CINIII组、宫颈癌组分别为12%(6/50)、16%(8/50)、42%(21/50),三组之间差异有统计学意义(P<0.05)。慢性宫颈炎~CINI组与CINII~CINIII组比较差异无统计学意义(P>0.05),CINII~CINIII组与宫颈癌组比较差异有统计学意义(P<0.05),慢性宫颈炎~CINI组与宫颈癌组比较差异有统计学意义(P<0.05)。h MLH1蛋白在宫颈癌的缺失表达显著高于癌前病变。h MSH2蛋白的缺失表达率在慢性宫颈炎~CINI组、CINII~CINIII组、宫颈癌组分别为4%(2/50)、6%(3/50)、12%(6/50),差异无统计学意义(P>0.05)。h MSH6蛋白的缺失表达率在慢性宫颈炎~CINI组、CINII~III组、宫颈癌组分别为2%(1/50)、4%(2/50)、10%(5/50),差异无统计学意义(P>0.05)。在宫颈癌组织中,h MLH1、h MSH2表达存在正相关关系(P<0.05),h MSH2、h MSH6表达存在正相关关系(P<0.05),h MLH1、h MSH6表达无明显相关关系(P>0.05)。结论错配修复基因h MLH1、h MSH2、h MSH6的缺失发生可能在宫颈癌的早期,联合检测三者有助于宫颈癌的早期诊断。
Objective To investigate the relationship between the expression of mismatch repair genes h MLH1, h MSH2 and h MSH6 and cervical lesions in the formation and development of cervical neoplasms. Methods Immunohistochemical SP method was used to detect the expression of mismatch repair genes h MLH1, h MSH2 and h MSH6 in 50 cases of chronic cervicitis to CINI group, 50 cases of CINII to CIN III group and 50 cases of cervical cancer group. Results The hMLH1 protein deletion rate was 12% (6/50), 16% (8/50), 42% (21/50) in CINI group, CINII-CINIII group and cervical cancer group The difference between the three groups was statistically significant (P <0.05). There was no significant difference between CINI group and CINII group (P> 0.05), but there was significant difference between CINII and CINIII group and cervical cancer group (P <0.05). Chronic cervicitis-CINI group There was significant difference between cervical cancer group (P <0.05). h MLH1 protein in cervical cancer was significantly higher than the loss of precancerous lesions. h The expression of MSH2 protein in chronic cervicitis-CINI group, CINII-CINIII group and cervical cancer group were 4% (2/50), 6% (3/50) and 12% (6/50) No statistical significance (P> 0.05). h The expression rate of MSH6 protein was 2% (1/50), 4% (2/50) and 10% (5/50) in CINI group, CINII-III group and cervical cancer group, respectively No statistical significance (P> 0.05). There was a positive correlation between hMLH1, h MSH2 expression and h MSH2, h MSH6 expression (P <0.05), h MLH1, h MSH6 expression in cervical cancer (P > 0.05). Conclusion The mismatch repair genes h MLH1, h MSH2, h MSH6 deletion may occur early in cervical cancer, joint detection of the three contribute to the early diagnosis of cervical cancer.