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目的:探讨哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)及其活化形式磷酸化mTOR(phos-phorylated mTOR,p-mTOR)在人大肠癌发生发展中的作用及临床意义。方法:应用组织芯片和免疫组织化学技术检测185例大肠癌组织及癌旁组织中mTOR及p-mTOR的表达,并分析其与大肠癌临床病理学特征如年龄、性别、肿瘤浸润深度(T分期)、淋巴转移、TNM分期和分化程度之间的关系。结果:在大肠癌旁组织中mTOR呈现弥散表达,p-mTOR基本不表达或散在表达,两者在大肠癌组织中的表达强度明显高于癌旁组织。mTOR和p-mTOR在大肠癌中的过表达率分别为45.9%和42.2%。两者均与性别、年龄无显著相关。mTOR的过表达与大肠癌的分化程度相关(P<0.05),而与浸润深度(T分期)、TNM分期、淋巴转移无显著相关(P>0.05)。p-mTOR的过表达与大肠癌的浸润深度(T分期)、淋巴转移和TNM分期显著相关(P<0.05),而与分化程度无显著相关(P>0.05)。结论:p-mTOR的过表达与人大肠癌的恶性表型密切相关,mTOR的磷酸化可能参与调控了大肠癌的发生发展。
Objective: To investigate the role of mammalian target of rapamycin (mTOR) and its activated form of phosphorylated mTOR (p-mTOR) in the development of human colorectal cancer and its clinical significance. Methods: The expression of mTOR and p-mTOR in 185 cases of colorectal cancer tissues and adjacent normal tissues were detected by tissue microarray and immunohistochemistry. The expressions of mTOR and p-mTOR in colorectal cancer tissues were analyzed with clinicopathological features such as age, sex, depth of tumor invasion ), Lymph node metastasis, TNM stage and the degree of differentiation. Results: The expression of mTOR in colorectal cancer tissues was diffusely expressed and p-mTOR was not expressed or scattered in colorectal carcinoma. The expression intensity of mTOR in colorectal cancer tissues was significantly higher than that in paracancerous tissues. Overexpression rates of mTOR and p-mTOR in colorectal cancer were 45.9% and 42.2%, respectively. Both were not significantly related to gender and age. The overexpression of mTOR was correlated with the degree of differentiation of colorectal cancer (P <0.05), but not with the depth of invasion (T stage), TNM stage and lymphatic metastasis (P> 0.05). The overexpression of p-mTOR was significantly associated with the depth of invasion (T stage), lymphatic metastasis and TNM stage (P <0.05), but not with the degree of differentiation (P> 0.05). Conclusion: The overexpression of p-mTOR is closely related to the malignant phenotype of human colorectal cancer. Phosphorylation of mTOR may be involved in the regulation of colorectal carcinogenesis.