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目的了解北京地区严重急性呼吸道感染病毒病原学现况,探讨不同年龄组、不同月份病毒检出情况,为预防策略制定、临床诊断、治疗提供病原学依据。方法采用多重RT-PCR法,对270份咽拭子样本同时检测腺病毒,偏肺病毒,冠状病毒,1型、2型、3型、4型副流感病毒,甲型、乙型流感病毒,甲型、乙型呼吸道合胞病毒,甲型鼻病毒,博卡病毒和肠道病毒共14种常见呼吸道病毒。结果 270份样本中检出阳性样本103份,阳性率为38.15%。其中HBo V的检出率最高,为46.68%。15岁~25岁组呼吸道病毒检出阳性率最高,为66.67%。各病毒不同月份检出情况普遍呈10月-次年4月检出数增高,5月-9月有零星的散发。结论 HBo V和Flu为北京地区严重急性呼吸道感染主要致病原,随年龄不同、流行季节中月份不同而有一定的流行规律。
Objective To understand the status of etiology of severe acute respiratory infection virus in Beijing and explore the detection of virus in different age groups and months and to provide etiological evidence for prevention strategy formulation, clinical diagnosis and treatment. Methods Multiplex RT-PCR method was used to detect adenovirus, Pneumovirus, coronavirus, parainfluenza virus type 1, type 2, type 3 and type 4 parainfluenza virus, type A and type B influenza virus, A, B respiratory syncytial virus, Rhinovirus, Boca virus and enterovirus total 14 kinds of common respiratory viruses. Results A total of 103 positive samples were detected in 270 samples, the positive rate was 38.15%. The detection rate of HBo V was the highest, 46.68%. The positive rate of respiratory virus was the highest in 15-year-old group of 25 years old, 66.67%. Detection of the virus in different months were generally October - the following year in April increased the number detected in May-September sporadic sporadic. Conclusions HBo V and Flu are the main pathogenic agents of severe acute respiratory infection in Beijing. With different ages and different months in the epidemic season, HBo V and Flu have certain epidemic patterns.