利用MATLAB软件编制程序,方便地从待测组分激发光谱和发射光谱得到△λ变化时模拟的三维同步荧光光谱图,在此基础上,通过简单的分析和直观观察,可以得到最优的△λ。本方法作为同步荧光分析中△λ优化的辅助工具,具有快速简便,信息量大的特点,同时对仪器的要求不高。以维生素B2和水杨酸钠为例,对方法的应用进行了说明。 Using MATLAB software to program, it is convenient to obtain the three-dimensional synchronous fluorescence spectrum when △ λ changes from the excitation and emission spectra of the tested components. On this basis, through simple analysis and visual observation, the optimal △ λ. This method, as an aid to △ λ optimization in simultaneous fluorescence analysis, has the characteristics of quick and easy, large amount of information, and low requirement on the instrument. Taking vitamin B2 and sodium salicylate as examples, the application of the method is described.