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Aim:To investigate the biological activity of human lens epithelial cells (HLEC)in producing inducible nitric oxide synthase (iNOS) and nitric oxide (NO),and toassesse the effect of diethyldithiocarbamate (DDC) on iNOS mRNA levels andexpression of NOS.Methods:The human lens epithelial cell line SRA 01/04 wasused in this experiment.Semi-quantitative reverse transcription polymerase chainreaction (RT-PCR) and Western blotting were used to detect,respectively,iNOSmRNA expression and protein production.Results:A costimulation by inter-feron gamma (IFN-γ) and lipopolysaccharide (LPS) was necessary for iNOS ex-pression in HLEC.The expression of iNOS was significantly reduced in a dose-dependent manner by adding DDC from 10 μmol/L to 1 mmol/L.Conclusion:The expression of iNOS in HLEC needs co-stimulation with IFN-γ and LPS and itis inhibited by DDC.
Aim: To investigate the biological activity of human lens epithelial cells (HLEC) in producing inducible nitric oxide synthase (iNOS) and nitric oxide (NO), and toassesse the effect of diethyldithiocarbamate (DDC) on iNOS mRNA levels an Norm. Methods: The human lens epithelial cell line SRA 01/04 was used in this experiment. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to detect, respectively, iNOS mRNA expression and protein production. Results: A costimulation by inter- feron gamma (IFN-γ) and lipopolysaccharide (LPS) was necessary for iNOS ex-pression in HLEC. The expression of iNOS was significantly reduced in a dose-dependent manner by adding DDC from 10 μmol / L to 1 mmol / L. Conlusion : The expression of iNOS in HLEC needs co-stimulation with IFN-γ and LPS and itis inhibited by DDC.