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为了研究阐明补体系统终末阶段ＭＡＣ形成的调控机制，我们首先分离制备了Ｃ５６．建立了一种简便、灵敏、快速的微量反应住溶血测定法。分析了人红细胞膜蛋白成份对补体ＭＡＣ形成的同源限制作用，证明红细胞膜上同时存在两种具有同源限制功能的蛋白成份（ｍｐ１８和ｍｐ６５），并经ＤＥＡＥ－纤维素ＤＥ－３２．Ｓｅｐｈａｒｏｓｅ６Ｂ层析及ＳＤＳ－ＰＡＧＥ制备纯化了ｍｐ１８。利用单克隆抗体技术首次在国内建立了两株抗人补体终末阶段同源限制因子的杂交瘤（２Ａ７和ｌＦ１０）．经抗原特异性及功能分析提示它们所针对的抗原分子具有相似的功能，但抗原性和分子量不同：２Ａ７－Ａｇ为１８～２２ＫＤａ．ｌＦ１０－Ａｇ为６５ＫＤａ。进一步应用免疫荧光流式细胞分析、促同源补体溶血试验、中和实验、Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ、免疫沉淀、ＰＩ－ＰＬＣ处理、亲和层析纯化、ＥＬＩＳＡ竞争抑制实验等手段全面系统地鉴定了２Ａ７抗原的细胞分布、抗原特异、分子特性及功能，表明２Ａ７与国外的ＣＤ５９命名抗体ＭＥＭ４３和ｌＦ５共同识别同一膜糖蛋白分子。该抗原分子以糖脂链（ＧＰＩ）锚固于细胞膜上，证明２Ａ７为一株抗ＣＤ５９的单克隆抗体。观测了２Ａ７和纯化的ＣＤ５９分子对同源?
In order to study the regulation mechanism of MAC formation during the terminal phase of the complement system, we first prepared C56 separately. A simple, sensitive and rapid microreaction assay for haemolysis was established. The homologous restriction of human erythrocyte membrane proteins on complement MAC formation was analyzed, demonstrating that there are two protein components with homologous restriction function (mp18 and mp65) on the erythrocyte membrane and DEAE-cellulose DE-32. Sepharose 6B chromatography and SDS-PAGE purified mp18. For the first time using monoclonal antibody technology in the country established two anti-human complement end stage homologous restriction factor hybridomas (2A7 and lF10). Antigenic specificity and functional analysis suggest that they are directed against antigen molecules with similar functions, but different antigenicity and molecular weight: 2A7-Ag is 18 ~ 22KDa. lF10-Ag is 65 kDa. Furthermore, immunofluorescence flow cytometry, homologous complement hemolysis assay, neutralization assay, Western blotting, immunoprecipitation, PI-PLC treatment, affinity chromatography purification and ELISA competitive inhibition assay were used to comprehensively and systematically identify the 2A7 antigen Cell distribution, antigen specificity, molecular characteristics and functions, indicating that 2A7 and foreign CD59 named antibodies MEM43 and lF5 together identify the same membrane glycoprotein molecules. The antigen molecule anchored to the cell membrane with glycolipid chain (GPI) proved that 2A7 is a monoclonal antibody against CD59. Observed 2A7 and purified CD59 molecules on homologous?