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目的:探讨何首乌饮对亚急性衰老大鼠卵巢细胞凋亡相关基因P53和Bcl-2/Bax蛋白表达的影响。方法:采用D-半乳糖连续腹腔注射致亚急性衰老大鼠模型,在造模后以何首乌饮灌胃作为延缓衰老组;造模后应用逆转录聚合酶链反应测定各组P53的mRNA水平;Western Bloting法检测卵巢细胞Bcl-2和Bax f蛋白的表达情况。结果:与正常对照组比较,D-半乳糖致衰老的模型组大鼠卵巢细胞中P53基因和Bax蛋白表达明显上升,Bcl-2蛋白表达显著下降,差异有统计学意义(P<0.01);何首乌饮延缓衰老组较模型组P53基因和Bax蛋白表达明显下降(P<0.01),并上调Bcl-2蛋白的表达,呈现量效关系,以何首乌饮高剂量组效果作用最佳。结论:何首乌饮方剂可能是通过调节卵巢细胞凋亡相关基因和蛋白表达,来延缓衰老大鼠卵巢细胞凋亡的发生。
Objective: To investigate the effects of Heshouwuyin on the expression of apoptosis-related genes P53 and Bcl-2 / Bax protein in subacute aging rats. Methods: The model of subacute aging rats was induced by continuous intraperitoneal injection of D-galactose. The gavage of Polygonum multiflorum Thunb was taken as delayed aging group after modeling. The mRNA level of P53 in each group was determined by reverse transcription-polymerase chain reaction (RT-PCR) Western Bloting was used to detect the expression of Bcl-2 and Bax f protein in ovarian cells. Results: Compared with the normal control group, the expression of P53 gene and Bax protein in ovarian cells and the expression of Bcl-2 in the model group were significantly decreased (P <0.01). Compared with model group, the content of P53 gene and Bax protein decreased significantly (P <0.01), and the expression of Bcl-2 protein increased. The dose-effect relationship was found. Conclusion: Polygonum multiflorum recipe can delay the apoptosis of ovarian cells in aging rats by regulating the expression of apoptosis-related genes and proteins in ovarian cells.