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目的 了解噪声对耳蜗抗氧化酶防御体系的影响。方法 雄性花色豚鼠 1 6只 ,体重2 50～ 30 0g,随机分为 2组 ,正常对照组和噪声暴露组 ,每组 8只。噪声暴露组接触中心频率为 4kHz的倍频程连续噪声 ,强度为 1 0 0dB(SPL) ,每天 8h ,连续 3d。于接触噪声前、噪声暴露结束后即刻测定听觉诱发脑干反应 (ABR) ,并测定活性氧 (ROS)水平和超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化物酶 (GSH Px)活力。结果 噪声暴露组的ROS水平为 (2 81 .2± 3 .5)U/mgpro ,正常对照组为 (2 73 .0± 3 .2 )U/mgpro,差异有显著性 (P <0 .0 5) ,SOD、CAT及GSH Px活力分别为 (2 0 6 .5±5 .1 )NU/mgpro、(47.0± 9.0 )U/gpro、(1 4 .1± 2 .5)U/mgpro,比正常对照组 [分别为 (2 2 1 .8± 4 .8)NU/mgpro、(60 .8± 9.9)U/gpro、(2 1 .1± 3 .1 )U/mgpro]明显降低 ,且差异有显著性 (P <0 .0 5)。结论 噪声可以损伤耳蜗的抗氧化酶防御体系
Objective To understand the effect of noise on cochlear anti-oxidase defense system. Methods A total of 16 male guinea pigs weighing 50 to 300 g were randomly divided into 2 groups: normal control group and noise exposure group, with 8 rats in each group. The noise exposure group was exposed to octave continuous noise with a center frequency of 4 kHz, with an intensity of 100 dB (SPL) for 8 h per day for 3 consecutive days. Before exposure to noise, auditory evoked brainstem response (ABR) was measured immediately after the noise exposure, and the levels of reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT) Peroxidase (GSH Px) activity. Results The level of ROS in the exposed group was (2 81.2 ± 3.5) U / mgpro and that in the normal control group was (2 73.0 ± 3.2) U / mgpro, the difference was significant (P <0. 0 (P <0.05). The activities of SOD, CAT and GSH Px were (20.6 ± 5 .1) NU / mgpro, 47.0 ± 9.0 U / gpro and 14.4 ± 2.5 U / Was significantly lower than that of the normal control group [(21.8 ± 4.8 vs NU / mgpro, (60.8 ± 9.9) U / gpro, (21.1 ± 3.1) U / mgpro] And the difference was significant (P <0.05). Conclusion Noise can damage the cochlear anti-oxidase defense system