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[目的]构建玉米通用表达载体,以期为利用转基因手段提高玉米对非生物胁迫的耐受性奠定基础。[方法]通过对现有的pGreen0229植物表达载体进行改造,构建含有CaMv35S启动子驱动的抗草胺膦筛选基因bar、可用于连接目的基因的玉米表达载体PGM-35Sbar,并通过花粉管通道法转化吉444玉米自交系。[结果]PGM-35Sbar玉米通用载体构建成功,转化玉米植株后,得到抗性植株14棵,经PCR检测其中有12棵为阳性植株。[结论]该研究为快速构建含有特定目的基因的玉米表达载体奠定了基础。
[Objective] The aim of the study was to construct a universal expression vector for maize, with a view to laying the foundation for improving the tolerance of maize to abiotic stress using transgenic means. [Method] The existing pGreen0229 plant expression vector was transformed to construct a bar-resistant CaMv35S promoter-driven barley carboxin phosphine gene bar, which was used to ligate the gene expression vector PGM-35Sbar and transformed by pollen tube pathway Ji 444 maize inbred lines. [Result] The PGM-35Sbar corn common vector was successfully constructed. After transformation of maize plants, 14 resistant plants were obtained. Twelve of them were positive plants by PCR. [Conclusion] The study laid the foundation for rapid construction of maize expression vector containing specific gene of interest.