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本研究发现用ConA活化的灭活自身T细胞皮下免疫C57BL/6J小鼠后,能够明显抑制小鼠淋巴瘤细胞株(EL-4)在体内生长,小鼠生存时间延长。对其作用机制研究发现,Th1型细胞因子分泌增加,而外周血调节性T细胞(Treg)数量减少,对效应性T细胞的抑制功能减弱。进一步分析Treg减少的研究表明:免疫后小鼠血清中抗CD25抗体增多,血清过继转移实验证实正常小鼠接受了免疫小鼠血清后其体内的CD4+CD25+Treg减少。因此,本研究提示用ConA活化的灭活自身T细胞免疫可以通过上调Th1细胞活性、降低Treg数量和功能而增强抗肿瘤的免疫反应,达到抑制肿瘤的生长效应。
This study found that ConA-activated inactivated T cells subcutaneously immunized C57BL / 6J mice, can significantly inhibit mouse lymphoma cell line (EL-4) in vivo growth, mouse survival time. The mechanism of action found that Th1-type cytokine secretion increased, while the number of peripheral blood Treg regulatory cells (Treg) decreased the inhibitory effect of effector T cells weakened. Further analysis of Treg reduction studies showed that: after immunization in mice serum anti-CD25 antibody increased serum adoptive transfer experiments confirmed that the normal mice received immunized mice serum CD4 CD25 Treg reduction in vivo. Therefore, the present study suggests that the activation of self T cell activation by ConA may increase the anti-tumor immune response by up-regulating the activity of Th1 cells, decreasing the number and function of Tregs, and thus inhibiting tumor growth.