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作者应用ｃ－ｆｏｓ、Ｒｂ蛋白抗体和微波炉抗原修复法对人慢性放射性皮肤溃疡进行免疫组织化学研究。结果显示：①ｃ－ｆｏｓ蛋白在人放射性皮肤溃疡阳性率为８４．０％。阳性部位主要见于增生的鳞状上皮细胞核、增生的纤维母细胞以及小血管内皮细胞、小动脉中层平滑肌细胞核，部分细胞浆内有阳性反应。②将ｃ－ｆｏｓ蛋白在表皮的检出情况及反应强度与表皮的增生程度列表分析，提示ｃ－ｆｏｓ蛋白在各增生表皮以至癌变组织阳性率有升高趋势。③Ｒｂ蛋白在正常皮肤检测不出，在病变组织的增生部位均有表达且表达的阳性强度在各增生组织以至溃疡癌变组织内无明显差异，其阳性部位与ｃ－ｆｏｓ蛋白分布基本一致。以上结果说明ｃ－ｆｏｓ、Ｒｂ蛋白异常性高表达可能与放射性溃疡反复发作、经久不愈，最后癌变的机制等因素有关。
The authors apply c-fos, Rb protein antibody and microwave antigen repair method for chronic radiation-induced skin ulcer immunohistochemistry. The results showed that: ①c-fos protein in human radioactive skin ulcer positive rate was 84.0%. Positive sites mainly found in hyperplastic squamous cell nuclei, hyperplastic fibroblasts and small vascular endothelial cells, smooth muscle cells in the middle of the arterioles, some positive cytoplasm reaction. ② The c-fos protein in the epidermis of the detection and response intensity and epidermal hyperplasia degree list analysis, suggesting that c-fos protein in all proliferative epidermis as well as the positive rate of cancerous tissue increased. ③Rb protein can not be detected in normal skin, both in the proliferative parts of the diseased tissue, and the positive expression intensity in the proliferative tissue and ulcerated cancerous tissue showed no significant difference. The positive part of the protein is consistent with the distribution of c-fos protein. The above results suggest that c-fos, Rb protein abnormally high expression may be repeated episodes of radiated ulcers, prolonged unhealed, and finally the mechanism of carcinogenesis and other factors.