本实验研究在国内首次成功地建立了角膜内皮细胞培养系统,培养的牛角膜内皮细胞在10—14天内达到生长密集状态,其细胞形态学特征与天然的角膜内皮细胞相类似。我们应用培养细胞进行二性霉素B的毒性研究。根据前房注射二性霉素B的剂量为20～40μg/次,把培养细胞分别暴露于67μg/ml和133μg/ml的二性霉素B溶液中12小时,结果发现前者对培养细胞有轻微的毒性,而后者则有严重的细胞毒性。提示临床作前房注射二性霉素B时应特别慎重。 The experimental study successfully established the corneal endothelial cell culture system for the first time in China. The cultured corneal endothelial cells reached a growth-dense state within 10-14 days, and their cell morphological characteristics were similar to those of natural corneal endothelial cells. We used cultured cells to study amphotericin B toxicity. According to the injection of amphotericin B anterior chamber dose of 20 ~ 40μg / time, the cultured cells were exposed to 67μg / ml and 133μg / ml of amphotericin B solution for 12 hours and found that the former cultured cells were mild Toxicity, while the latter has serious cytotoxicity. Tips for clinical injection of amphotericin B anterior chamber should be particularly cautious.