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[目的]探讨葛根素注射液对阿霉素所致大鼠心肌损伤保护作用及其机制。[方法]SD雄性大鼠随机分为5组(每组12只):①阿霉素(ADM)组:腹腔注射ADM,每次2.5mg/kg,每周1次,共用药10周;②对照组:用相同容量的生理盐水代替ADM,用法同ADM组;③葛根素低、中、高剂量组(共3组):ADM用药方法同ADM组,同时经腹腔分别注射15mg/kg、30mg/kg、60mg/kg,每天1次,连续用药10周。10周末测量各组大鼠心功能及血流动力学指标;测定各组大鼠血清门冬氨酸氨基转移酶活性的测定、心肌肌酸激酶活性以及心肌超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。[结果]与对照组相比,阿霉素组大鼠心功能显著降低(P﹤0.01)、SOD活性显著下降(P﹤0.01)、MDA含量显著增加(P﹤0.01);与阿霉素组相比,葛根素高中低剂量组心功能显著降低(P﹤0.01)、SOD活性显著提高(P﹤0.01)、MDA含量显著下降(P﹤0.01)。[结论]葛根素可通过降低阿霉素对大鼠心脏的氧化损伤,而起到对阿霉素所致心肌损伤的保护作用。
[Objective] To explore the protective effect and mechanism of puerarin on doxorubicin-induced myocardial injury in rats. [Methods] SD male rats were randomly divided into 5 groups (12 in each group): ① Adriamycin (ADM) group: intraperitoneal injection of ADM 2.5mg / kg once a week for 10 weeks; ② Control group: the same volume of saline instead of ADM, with the ADM group; ③ puerarin low, medium and high dose group (3 groups): ADM drug group with ADM, while intraperitoneal injection of 15mg / kg, 30mg / kg, 60mg / kg, 1 times a day for 10 weeks. The cardiac function and hemodynamic indexes of rats in each group were measured at the end of 10 weeks. The serum activities of aspartate aminotransferase, activity of myocardial creatine kinase and activity of superoxide dismutase (SOD) Malondialdehyde (MDA) content. [Results] Compared with the control group, the cardiac function of adriamycin group was significantly decreased (P <0.01), while the SOD activity was significantly decreased (P <0.01) and MDA content was significantly increased (P <0.01) (P <0.01), SOD activity increased significantly (P <0.01) and MDA content decreased significantly (P <0.01). [Conclusion] Puerarin can protect against doxorubicin-induced myocardial injury by decreasing the oxidative damage induced by doxorubicin in rat heart.