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目的 探讨谷胱苷肽硫转移酶M1(glutathioneS -transferaseM 1,GSTM 1)和T1(glutathioneS -transferaseT1,GSTT1)基因多态性与女性肺癌遗传易感性的关系。方法 采用病例 -对照研究方法和多重PCR技术检测女性肺癌病例组 4 2人和健康对照组 5 5人的GSTM1和GSTT1基因缺陷型的频率 ,并评价GSTM1和GSTT1基因型以及他们之间的交互作用与肺癌遗传易感性的关系。结果 在本次研究的人群中 ,病例组GSTM1和GSTT1基因缺陷型的频率分别为 6 6 7%和 4 5 2 % ,对照组为 5 4 5 %和 38 2 %。GSTM1基因缺陷型和GSTT1基因缺陷型的频率在病例组和对照组之间均无显著性差异 (P >0 0 5 )。在不吸烟的女性人群中 ,GSTM 1基因缺陷型携带者患肺癌的危险性是GSTM1基因功能型携带者的 2 5 5 7倍 (P =0 0 4 6 ) ;GSTT1基因缺陷型则与女性肺癌的发生无显著关联 (P =0 5 5 7)。此外 ,GSTT1基因型与GSTM1基因型之间亦无明显的交互作用 (P >0 0 5 )。结论 GSTM 1基因缺陷型可能是非吸烟女性患肺癌的重要危险因素 ,GSTT1基因缺失则可能与肺癌的发生无关。在女性肺癌的发生过程中GSTM 1与GSTT1可能不存在交互作用。
Objective To investigate the relationship between genetic polymorphisms of glutathione S -transferase M1 (GSTM1) and glutathione S -transferase T1 (GSTT1) and genetic susceptibility of female lung cancer. Methods The frequency of GSTM1 and GSTT1 gene deficiencies in 42 women with lung cancer and 54 healthy controls were detected by case-control study and multiplex PCR. The genotypes of GSTM1 and GSTT1 and their interaction were also evaluated And genetic susceptibility to lung cancer. Results In this study population, the frequencies of GSTM1 and GSTT1 gene-defective cases were 66.7% and 45.2% in the case group and 54.5% and 38.2% in the control group, respectively. There was no significant difference between the case group and the control group in the frequency of GSTM1-deficient and GSTT1-deficient (P> 0.05). Among non-smoking female population, the risk of lung cancer in GSTM1-deficient carriers was 257 times more than that of GSTM1-functional carriers (P = 0.046); GSTT1-deficient carriers were associated with lung cancer in women No significant correlation was found between the two groups (P = 0 5 5 7). In addition, there was no significant interaction between GSTT1 genotype and GSTM1 genotype (P> 0.05). Conclusion Defective GSTM1 gene may be an important risk factor for lung cancer in non-smokers and the absence of GSTT1 gene may be associated with lung cancer. There may be no interaction between GSTM1 and GSTT1 during the development of female lung cancer.