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以富勒烯C_(60)作为聚苯胺的导电掺杂剂,采用乳液聚合-复合法制备了聚乳酸/聚苯胺/富勒烯复合体系(PLA81/PANI14/C_(60)5),并对其结构和性能进行了表征与理论分析.通过静电纺丝-编织法制备了PLA/PANI/C_(60)导电纤维管.利用555定时器原理设计自制了多谐振荡信号的电刺激装置,将其用于导电纤维管的细胞培养,以考察电刺激作用下导电纤维管上成骨细胞的生长和增殖情况.结果表明,PLA/PANI/C_(60)薄片的电阻率为104Ω·cm数量级;C_(60)能够促进苯胺乳液聚合的链增长,提高聚苯胺产物的聚合度;PLA/PANI/C_(60)纤维管形貌规整,具有良好的亲水性;在18μA/1 Hz/500 ms电刺激的作用下,PLA/PANI/C_(60)纤维对成骨细胞的生长具有明显的促进作用.加载适当电流电刺激能够加强生长因子对成骨细胞的分化作用,有利于促进成骨细胞的生长和繁殖.
Polylactic acid / polyaniline / fullerene composites (PLA81 / PANI14 / C_ (60) 5) were prepared by emulsion polymerization-complex method using fullerene C 60 as the conductive dopant for polyaniline. The structure and properties of PLA / PANI / C_ (60) conductive fiber were prepared by electrospinning-weaving method.The electrical stimulation device with multi-harmonic oscillation signal was designed by using 555 timer principle, The results showed that the resistivity of PLA / PANI / C_ (60) sheet was in the order of 104 Ω · cm, and the electrical conductivity of PLA / PANI / C 60 could promote the chain growth of aniline emulsion polymerization and increase the degree of polymerization of polyaniline product. The morphology of PLA / PANI / C 60 fiber tube was well-ordered and hydrophilic. At 18μA / 1 Hz / 500 ms Under the stimulation of electrical stimulation, the PLA / PANI / C 60 fibers promoted the growth of osteoblasts significantly.Adding appropriate current electrical stimulation could enhance the differentiation of osteoblasts by growth factors and promote the proliferation of osteoblasts Growth and reproduction.