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目的:建立HPLC同时测定脱脂紫苏子中迷迭香酸和木犀草素含量的方法。方法:采用Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.1%磷酸溶液(55∶45),流速1 m L·min-1,检测波长330,350 nm,柱温30℃。结果:迷迭香酸在9.91~158.55 mg·L-1回归方程为Y=108.83X+0.14(r=0.999 9),平均回收率为99.68%,RSD 2.01%;木犀草素在0.997~15.952 mg·L-1回归方程为Y=119.63X-3.68(r=0.999 9),平均回收率为100.34%,RSD 2.86%。结果:该方法操作简便、准确,重复性良好,可用于测定脱脂紫苏子中迷迭香酸和木犀草素的含量。
Objective: To establish a method for simultaneous determination of rosmarinic acid and luteolin in non-fat Perilla frutescens L. by HPLC. METHODS: Diamonsil C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was methanol-0.1% phosphoric acid solution (55:45) and the flow rate was 1 m L · min-1. The detection wavelength was 330 and 350 nm. Results: The regression equation of rosmarinic acid at 9.91 ~ 158.55 mg · L-1 was Y = 108.83X + 0.14 (r = 0.999 9) with the average recovery of 99.68% and RSD 2.01%, and the luteolin at 0.997 ~ 15.952 mg · The regression equation of L-1 was Y = 119.63X-3.68 (r = 0.999 9) with the average recovery of 100.34% and RSD of 2.86%. Results: The method was simple, accurate and reproducible. It could be used to determine the content of rosmarinic acid and luteolin in the defatted perilla.