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为探讨chTERT mRNA表达及端粒酶活性在LaCl3诱导MDCC-MSB1细胞凋亡中的作用。将肿瘤细胞常规培养于RPMI1640培养液中,加入终浓度为3mmol/L的LaCl3共培养,分别于培养第12、24、36和48h,用透射电镜观察LaCl3致MDCC-MSB1细胞的形态学变化,应用流式细胞仪检测细胞凋亡,以TRAP-PCR银染法检测端粒酶活性,以实时荧光定量PCR法检测chTERT基因mRNA的表达量变化。结果显示,3mmol/L的LaCl3作用第12、24、36和48h,透射电镜和流式细胞仪法均检测到明显的细胞凋亡变化,端粒酶活性下降,chTERT mRNA的表达量下降,并呈时间-效应关系。证实,LaCl3可通过降低chTERT mRNA的表达量抑制MDCC-MSB1细胞的端粒酶活性而诱导其发生凋亡。
To investigate the role of chTERT mRNA expression and telomerase activity in LaCl3-induced apoptosis of MDCC-MSB1 cells. The tumor cells were routinely cultured in RPMI1640 medium, and LaCl3 was added to a final concentration of 3mmol/L for co-culture. At 12, 24, 36 and 48 hours after culture, the morphological changes of MDCC-MSB1 cells induced by LaCl3 were observed by transmission electron microscopy. Apoptosis was detected by flow cytometry. The activity of telomerase was detected by TRAP-PCR silver staining. The mRNA expression of chTERT gene was detected by real-time fluorescence quantitative PCR. The results showed that at 12th, 24th, 36th, and 48th hour after 3mmol/L LaCl3 treatment, significant apoptosis was detected by transmission electron microscopy and flow cytometry. The activity of telomerase was decreased and the expression of chTERT mRNA was decreased. Time-effect relationship. It was confirmed that LaCl3 can induce the apoptosis of MDCC-MSB1 cells by inhibiting the telomerase activity of MDCC-MSB1 cells by decreasing the expression of chTERT mRNA.