近年来,人们试图用无血清或无蛋白培养基来培养致病性钩端螺旋体(以下简称钩体).吐温在培养基中作为钩体代谢的碳源.市售的吐温含有未酯化的脂肪酸、聚乙二醇和其他副产品,这些物质能阻碍钩体的生长发育.本文作者报告了用分溶方法对吐温40、60和80进行脱毒处理,及钩体在用脱毒吐温替代炭处理吐温的Bey和Johnson全综合无蛋白培养基中的生长情况. In recent years, attempts have been made to develop pathogenic Leptospira (hereinafter referred to as the Leptospira) in serum-free or protein-free media Tweens act as a carbon source for metabolism of the hook body in the media Commercially available Tweens contain non-ester These fatty acids, polyglycols, and other by-products can hinder the growth and development of the leptospira.The authors report the detoxification of Tween 40, 60 and 80 by the method of dissolution, Temperature-Surrogate Carbon Treatment Tween’s Bey and Johnson Total Synthetic Protein-Free Media Growth.