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分析尿中存在的药物通常要求首先从高浓度的内源代谢化合物中提取。通过用与氰予柱结合的环代替注射环，尿可以被直接注射到高效液相色谱仪。当阀门在负荷位置时，干扰物就从予柱上洗除，当阀门转向注入位置时，流动相从子柱将可卡因洗出到分析柱上。用这种技术可以初步检验出可卡因和本酰芽子碱。这项技术要求用 １００ｕｌ的样品，检出极限为 １０ｎｇ／ｍｌ。
Analysis of drugs present in urine generally requires first extraction from high concentrations of endogenous metabolites. Urine can be injected directly into the HPLC by replacing the injection loop with a ring that binds to the column. When the valve is in the load position, the interfering substance is removed from the pre-column. When the valve is turned to the injection position, the mobile phase elutes cocaine from the sub-column onto the analytical column. Using this technique, cocaine and the aconitine can be initially tested. This technique requires a 100ul sample with a detection limit of 10ng / ml.