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目的 研究端粒酶mTERT基因在不同年龄SD鼠睾丸组织内的表达及其意义。方法 应用原位分子杂交技术 ,对 11个年龄组 (每组 5只 )健康雄性SD鼠睾丸组织中端粒酶mTERT基因进行检测和定位 ,并运用图像分析系统对mTERT表达水平进行研究。结果 端粒酶mTERT基因在SD鼠睾丸组织内的阳性表达率为 98% (5 4/ 5 5 ) ,其阳性信号表达于A、B型精原细胞、初级精母细胞、次级精母细胞和精子细胞的胞浆内 ,A型精原细胞的表达水平最高 ,与其他类型细胞相比差异有统计学意义 (P <0 .0 5 ) ;在间质细胞、支持细胞等非生殖细胞和精子中均无端粒酶mTERT基因的表达。结论 本研究提示端粒酶mTERT基因的表达与雄性生殖细胞的端粒酶活性表达一致 ,其能否成为雄性生殖调控的新的基因靶点尚待进一步研究。
Objective To study the expression of telomerase mTERT gene in testes of SD rats of different ages and its significance. Methods The in situ hybridization technique was used to detect and locate the telomerase telomerase (mTERT) gene in testicular tissues from healthy male SD rats of 11 age groups (5 rats in each group). The expression of mTERT was analyzed by image analysis system. Results The positive expression rate of telomerase mTERT gene in SD rat testis was 98% (5 4/5 5). The positive expression of mTERT gene in A, B spermatogonia, primary spermatocyte, secondary spermatocyte And sperm cells in the cytoplasm, the highest expression level of type A spermatogonia, compared with other types of cells, the difference was statistically significant (P <0. 05); in stromal cells, supporting cells and other non-germ cells and No sperm telomerase mTERT gene expression. Conclusions This study suggests that the expression of telomerase mTERT gene is consistent with the expression of telomerase activity in male germ cells. Whether it can become a new gene target for male reproductive regulation remains to be further studied.