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目的探讨不同比例的n-6/n-3多不饱和脂肪酸(PUFA)对N-甲基亚硝基脲(MNU)诱导的乳腺癌大鼠乳腺组织中雌激素受体(ER)和p53表达的影响。方法雌性SD大鼠随机分为n-6 PUFA、10∶1 n-6/n-3、5∶1n-6/n-3、1∶1 n-6/n-3和正常对照5组,前4组以50mg/(kg.bw)MNU单次腹腔注射诱导乳腺肿瘤发生,正常对照组注射等体积无菌生理盐水。给药后立即分组喂养不同饲料,在8和18周时处死动物,RT-PCR和蛋白印迹(W estern b lot)技术检测各组大鼠乳腺组织ER和p53表达。结果4组乳腺癌大鼠乳腺组织中ER和p53的表达均较正常对照组〔(0.73±0.11),(0.08±0.01)〕有所升高,其中n-6组最高〔(1.32±0.18),(1.43±0.56)〕,其余各组随n-6/n-3比值减小而下降,1∶1 n-6/n-3组〔(0.95±0.12),(0.12±0.06)〕显著低于n-6组(P<0.05)。结论不同比例n-6/n-3多不饱和脂肪酸对MNU诱导的乳腺癌大鼠乳腺组织ER和p53的表达具有不同影响,1∶1 n-6/n-3膳食脂肪酸构成能有效抑制乳腺癌大鼠乳腺组织ER和p53表达的升高。
Objective To investigate the effects of different proportions of n-6 / n-3 polyunsaturated fatty acids (PUFA) on the expression of estrogen receptor (ER) and p53 in mammary gland tissues of N-methylnitrosourea (MNU) -induced breast cancer rats Impact. Methods Female Sprague-Dawley rats were randomly divided into 5 groups: n-6 PUFA, 10:1 n-6 / n-3, 5:1 n-6 / n-3, The first 4 groups were given intraperitoneal injection of 50mg / (kg.bw) MNU to induce mammary tumor, and the normal control group was injected with equal volume of sterile saline. Animals were sacrificed at 8 and 18 weeks immediately after administration of different diets. The expression of ER and p53 in breast tissues of rats in each group was detected by RT-PCR and Western blotting. Results The expression of ER and p53 in mammary gland tissues of 4 groups of breast cancer patients were significantly higher than those in normal control group (0.73 ± 0.11, 0.08 ± 0.01), the highest in n-6 group (1.32 ± 0.18) , (1.43 ± 0.56)], while the other groups decreased with the decrease of n-6 / n-3 ratio (0.95 ± 0.12, 0.12 ± 0.06) in 1:1 n-6 / n-3 group Lower than n-6 group (P <0.05). Conclusion Different proportions of n-6 / n-3 polyunsaturated fatty acids have different effects on the expression of ER and p53 in MNU-induced breast cancer rats. The dietary fatty acid composition of 1: 1 n-6 / n-3 can effectively inhibit the expression of breast cancer Increased expression of ER and p53 in breast cancer of rats.