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目的探讨SEL1L(human Sel-1-like)mRNA及其蛋白在食管癌组织中的表达及其临床意义。方法应用免疫组织化学SP法检测90例手术切除的食管鳞状细胞癌、35例距癌灶边缘5 cm以上切缘的正常黏膜、60例癌旁黏膜及20例内窥镜活检的食管鳞状上皮不典型增生组织中SEL1L蛋白的表达;运用原位分子杂交技术检测上述癌组织、正常黏膜、癌旁黏膜中SEL1LmRNA的表达。结果(1)SEL1L mRNA在食管鳞状细胞癌的表达率为80.0%(72/90),较正常黏膜的14.3% (5/35)和癌旁黏膜的16.7%(10/60)高(P<0.01);SEL1LmRNA在有淋巴结转移组的表达阳性率为92.7%(38/41)比无淋巴结转移组69.4%(34/49)高(P<0.01)。(2)SEL1L蛋白在鳞状细胞癌的表达阳性率为87.8%(79/90),在鳞状上皮不典型增生中的表达阳性率为90.0%(18/20),分别较正常黏膜的14.3%(5/35)和癌旁黏膜的13.3%(8/60)高(P<0.01)。SEL1L蛋白表达与患者性别、年龄、肿瘤位置、大小、分化程度、浸润深度、淋巴结转移及临床分期均无明显相关性(P>0.05)。(3)食管鳞状细胞癌组织中SEL1LmRNA和SEL1L蛋白的表达呈明显正相关(P=0.492,P<0.01)。结论(1)SEL1L蛋白表达的调控主要在转录水平,SEL1L蛋白表达水平的升高主要是相应转录水平上调的结果。(2)SEL1L蛋白过表达可能是食管鳞状细胞癌发生的早期表现,SEL1L蛋白的检测可作为识别食管癌高风险患者的生物标记物。
Objective To investigate the expression of SEL1L (human Sel-1-like) mRNA and its protein in esophageal carcinoma and its clinical significance. Methods 90 cases of esophageal squamous cell carcinoma were resected by immunohistochemistry SP method. 35 cases of normal mucosa from the margin of 5 cm or more, 60 cases of adjacent mucosa and 20 cases of endoscopic biopsy esophageal squamous cell carcinoma The expression of SEL1L protein in epithelial dysplasia tissues was detected by using in situ hybridization. The expression of SEL1L mRNA in the above cancerous tissue, normal mucosa and paracancerous mucosa was detected by in situ hybridization. Results (1) The expression of SEL1L mRNA in esophageal squamous cell carcinoma was 80.0% (72/90), higher than that in normal mucosa (5/35) and adjacent mucosa (16.7%, P <0.01). The positive rate of SEL1L mRNA expression in lymph node metastasis group was 92.7% (38/41), which was higher than that in non-lymph node metastasis group (69.4%, 34/49) (P <0.01). (2) The positive rate of SEL1L protein in squamous cell carcinoma was 87.8% (79/90), and the positive rate in squamous cell dysplasia was 90.0% (18/20), which was respectively 14.3 % (5/35) and adjacent mucosa 13.3% (8/60) (P <0.01). There was no significant correlation between SEL1L protein expression and gender, age, tumor location, size, differentiation, depth of invasion, lymph node metastasis and clinical stage (P> 0.05). (3) The expression of SEL1L mRNA and SEL1L protein in esophageal squamous cell carcinoma was positively correlated (P = 0.492, P <0.01). Conclusion (1) The regulation of SEL1L protein expression is mainly at the transcriptional level, and the increase of SEL1L protein expression is mainly the result of the corresponding transcriptional level upregulation. (2) SEL1L protein overexpression may be an early manifestation of esophageal squamous cell carcinoma, SEL1L protein detection can be used as a biomarker to identify patients with high esophageal cancer risk.