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以20个新育成的粳稻BT型不育系和10个恢复系为材料,采用SSR分子标记和表型性状2种聚类方法对杂交粳稻亲本的遗传多样性进行比较研究,并分析这些亲本的遗传差异。结果表明,64对SSR引物在30个亲本中共检测到185个多态性片段,平均每对引物为2.9个,以第11染色体上的平均等位基因数目最多,每个SSR位点的多态性信息含量指数(PIC值)变化范围为0.064～0.844。利用SSR分子标记将30个亲本材料划分为7大类群,其中所有不育系被划分为一群,分类结果与系谱分析基本吻合。表型聚类以10个农艺性状为依据,将供试材料划分为4大类群,不育系和恢复系被聚到不同的类群,群内差异不大。同表型性状聚类相比,SSR分子标记聚类能更准确的揭示亲本之间的遗传差异,是研究亲本遗传多样性的一种较好方法。
Twenty newly-developed japonica BT lines and 10 restorer lines were used to study the genetic diversity of parents of japonica hybrid rice using two kinds of SSR markers and phenotypic traits. Genetic differences. The results showed that 64 SSR primers detected 185 polymorphic fragments in 30 parents, with an average of 2.9 pairs per primer. The average number of alleles per chromosome was 11, and the polymorphism of each SSR locus Sexual information content index (PIC) ranged from 0.064 to 0.844. Thirty parents were divided into seven groups using SSR markers. All the sterile lines were divided into one group, and the classification results were basically consistent with pedigree analysis. Phenotypic clustering was based on 10 agronomic traits. The tested materials were divided into four groups, and sterile and restorer lines were clustered into different groups with little difference in the group. Compared with phenotypic clustering, SSR molecular marker clustering can reveal the genetic difference between parents more accurately, which is a better way to study the genetic diversity of parents.