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目的在猪模型中前瞻性研究此假说:具有商用校准微球体的肥胖栓塞可以在8周内明显抑制全身胃饥饿素水平并影响体质量增加。材料与方法本研究经实验动物管理委员会批准。对12头健康生长猪(体质量30.3～47.0kg,平均38.4kg)进行研究。选择性对供应胃底的胃动脉注入40μm校准微球体行肥胖栓塞。6只猪行肥胖栓塞,另6只猪则行盐水注入作为对照组。在基线及1～8周获得动物体质量、空腹血浆胃饥饿素及葡萄糖水平。分析每个时间点的血浆胃饥饿素水平及体质量差异,Wilcoxon符号秩检验分析组内差异,Wilcoxon秩和检验分析组间差异。结果对照组与实验组的胃饥饿素水平随时间变化的形式有显著差异。测量对照组及实验组胃饥饿素周水平作为以基线为参考的变化值。对照组猪注入盐水后平均胃饥饿素值增加了(328.9±129.0)pg/dL,而实验组则降低了(537.9±209.6)pg/dL(P=0.004)。对照组与实验组的体质量随时间变化的形式有显著差异。实验组栓塞后平均体质量的增加少于对照组[分别为(3.6±3.8)kg∶(9.4±2.8)kg,P=0.025]。结论肥胖栓塞可以显著抑制胃饥饿素并影响体质量增加。在此技术常规应用于人之前,需要进一步的研究。
Objective To prospectively study this hypothesis in a porcine model: Obesity embolization with commercially available microspheres significantly inhibited whole body ghrelin levels and affected body mass gain within 8 weeks. Materials and Methods The study was approved by the Laboratory Animal Management Committee. 12 healthy growing pigs (body weight 30.3 ~ 47.0kg, an average of 38.4kg) were studied. Selective injection of 40 μm calibrated microspheres into the gastric artery supplying gastric fundus obese. Six pigs were obese and the other six pigs were injected with saline as the control group. Body weight, fasting plasma ghrelin and glucose levels were obtained at baseline and from 1 to 8 weeks. Plasma ghrelin levels and body weight differences were analyzed at each time point. Wilcoxon signed-rank test was used to analyze intra-group differences. Wilcoxon rank-sum test was used to analyze differences between groups. Results There was a significant difference in the form of ghrelin between the control group and the experimental group over time. The levels of ghrelin in the control and experimental groups were measured as baseline-referenced changes. The average ghrelin value of the control group increased (328.9 ± 129.0) pg / dL after saline injection, while the experimental group decreased (537.9 ± 209.6) pg / dL (P = 0.004). There was significant difference between the control group and the experimental group in the form of body mass with time. The mean increase in body mass after embolisation in the experimental group was less than in the control group (3.6 ± 3.8 kg: 9.4 ± 2.8 kg, P = 0.025, respectively). Conclusion Obese embolism can significantly inhibit ghrelin and affect body mass gain. Before this technique is routinely applied to people, further research is needed.