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目的阐明三尖杉酯碱（HT）作用于K562细胞的机制。方法应用细胞形态、DNA凝胶电泳和流式细胞仪等检测,观察HT对K562细胞株的作用方式,进一步用RT-PCR方法研究bcr/abl基因在转录水平的改变。结果0.01～100.00μg/ml浓度HT可诱导K562细胞凋亡,并呈时间和剂量依赖性。随着药物作用时间的延长,bcr/abl基因的转录下调。结论低浓度HT就可通过抑制bcr/abl基因的表达,诱导K562细胞凋亡。
Objective To elucidate the mechanism of action of harringtonine (HT) on K562 cells. Methods Cell morphology, DNA gel electrophoresis and flow cytometry were used to detect the effect of HT on K562 cells. The transcription level of bcr / abl gene was further studied by RT-PCR. Results The concentration of 0.01 ~ 100.00μg / ml HT could induce the apoptosis of K562 cells in a time and dose dependent manner. With prolonged drug action, transcription of the bcr / abl gene was down-regulated. Conclusion Low concentration of HT can induce the apoptosis of K562 cells by inhibiting the expression of bcr / abl gene.