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目的观察二甲基亚砜(DMSO)、全反式维甲酸(ATRA)和9顺式维甲酸(9cisRA)对人胆管癌细胞株QBC939的作用,筛选对它有效的分化诱导剂。方法以DMSO、ATRA和9cisRA作用于培养的胆管癌细胞株,描绘生长曲线,观察细胞形态,双层软琼脂培养法观察细胞恶性度,流式细胞术(FCM)检测细胞周期动力学变化。结果10-6mol/L9cisRA作用2d后开始抑制QBC939细胞生长,抑制率为39.4%～80.7%(P<0.05)。细胞软琼脂集落形成能力较对照组下降80.9%(P<0.01)。细胞形态向正常细胞转化。9cisRA作用1、3、5、7dG0/G1期细胞比例分别为55.56%、63.92%、73.38%、76.92%。ATRA对QBC939细胞无明显作用,DMSO主要引起细胞死亡。结论9cisRA可诱导QBC939细胞向良性表型分化,对该细胞株具有有诱导分化作用。
Objective To observe the effect of dimethylsulfoxide (DMSO), all-trans retinoic acid (ATRA) and 9-cis retinoic acid (9cisRA) on human cholangiocarcinoma cell line QBC939 and to screen for effective differentiation inducer. Methods The cultured cholangiocarcinoma cell lines were treated with DMSO, ATRA and 9cisRA, and the growth curve was drawn. The cell morphology was observed. The cell malignancy was observed by double soft agar culture. The cell cycle kinetics were detected by flow cytometry (FCM). Results 10-6mol / L 9cisRA began to inhibit the growth of QBC939 cells 2d later, with the inhibition rate of 39.4% -80.7% (P <0.05). The colony forming ability of soft agar was decreased by 80.9% (P <0.01) compared with the control group. Cell morphology is transformed into normal cells. The cell percentage of 9cisRA at 1, 3, 5, 7dG0 / G1 phase were 55.56%, 63.92%, 73.38% and 76.92%, respectively. ATRA had no significant effect on QBC939 cells, DMSO mainly caused cell death. Conclusion 9cisRA can induce QBC939 cells to differentiate into benign phenotype, which can induce differentiation of this cell line.