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目的以7月份和12月份采收丹参茎叶的水提物和醇提物为研究对象,评价丹参茎叶提取物的抗氧化效应,并探讨丹参茎叶提取物抗氧化活性的物质基础。方法采用超高效液相-三重四级杆质谱联用技术(UPLC-TQ/MS)对各提取物中化学成分进行定性定量分析,明确丹参茎叶提取物中主要丹酚酸类化合物(丹参素、咖啡酸、迷迭香酸和丹酚酸B),基于1,1-二苯基-2-三硝基苦肼(DPPH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基清除法和铁还原/抗氧化能力(FRAP)法对丹参茎叶提取物进行抗氧化活性评价,同时以市售丹参(根及根茎)样品作对照。结果 7月份采收丹参茎叶水提物(SY-7)抗氧化活性最强,且总酚酸的量最高(75.663 mg/g),其次为7月份采收丹参茎叶醇提物(CY-7),市售丹参提取物抗氧化活性及总酚酸的量均低于7月份采收丹参茎叶提取物。丹酚酸单体化合物丹参素、咖啡酸、迷迭香酸和丹酚酸B均具有明显抗氧化、清除自由基能力,且表现出明显的量效关系。结论丹参茎叶水提物和醇提物具有较强的体外抗氧化活性,且含有丰富的丹酚酸类化合物,其中含有的丹参素、咖啡酸、迷迭香酸和丹酚酸B具有明显抗氧化活性。
OBJECTIVE To evaluate the anti-oxidative effects of stem and leaf extracts of Salvia miltiorrhiza and Salvia miltiorrhiza stems and leaves, and to study the material basis of antioxidative activity of Salvia miltiorrhiza stem and leaf extracts in July and December. Methods The chemical constituents of the extracts were characterized by UPLC-TQ / MS. The main components of salvianolic acids (Danshensu Caffeic acid, rosmarinic acid and salvianolic acid B) based on 1,1-diphenyl-2-trinitrohydrazide (DPPH), 2,2-azino-bis Thiazole-6-sulfonic acid) diammonium salt (ABTS) free radical scavenging assay and iron reduction / antioxidant capacity (FRAP) method were used to evaluate the antioxidant activity of Salvia miltiorrhiza stem and leaf extracts. ) Sample as a control. Results The water extract of Salvia miltiorrhiza stems and leaves (SY-7) was the strongest in July with the highest content of total phenolic acids (75.663 mg / g), followed by the recovery of ethanol extract from stems and leaves of Salvia miltiorrhiza in July -7). The anti-oxidant activity and the total phenolic acids of commercially available salvia miltiorrhiza extracts were lower than those of the salvia miltiorrhiza stem and leaf extracts in July. Salviae miltiorrhizin, caffeic acid, rosmarinic acid and salvianolic acid B of salvianolic acid monomer compounds all showed obvious antioxidant and scavenging ability of free radical, and showed a significant dose-response relationship. Conclusion Water and ethanol extracts of stem and leaf of Salvia miltiorrhiza Bunge have strong antioxidant activity in vitro, and are rich in salvianolic acids, including Danshensu, caffeic acid, rosmarinic acid and salvianolic acid B Antioxidant activity.