采用显微注射法将绿色荧光蛋白 (GFP)基因重组表达质粒导入金鱼受精卵中 ,以期获得能发绿色荧光的金鱼。结果显示 ,在注射的 3批金鱼受精卵中 (第一批 4 310粒 ,第二批 395 2粒 ,第三批 4 0 5 6粒 ) ,分别孵出鱼苗 5 4 3、2 82和 2 6 6尾 ,出苗率为 17.0 2 %、12 .5 3%和 13.5 2 % ,其中表达绿色荧光的金鱼分别为 2 3、2 4和18尾 ,表达率为 4 .2 4 %、8.5 1%和 6 .77%。荧光表达检测发现 ,从肌肉效应期就开始检测得到绿色荧光 The green fluorescent protein (GFP) gene recombinant plasmid was introduced into goldfish zygotes by microinjection to obtain green fluorescent goldfish. The results showed that among the three batches of goldfish zygotes injected (4 310 first batches, 395 2 second batches and 405 6 third batches), fry were hatched at 5 4 3, 2 82 and 2 6, respectively 6, the emergence rate was 17.0 2%, 12.53% and 13.52% respectively. The goldfish expressing green fluorescence were 23, 24 and 18 respectively, the expression rates were 4.24% and 8.51% 6 .77%. Fluorescent expression test found that from the muscle effect began to detect green fluorescence