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目的从野生蟾蜍分离致病性着色真菌。方法在着色芽生菌病高发区和无病区分别捕捉野生蟾蜍,取内脏制成悬液分离着色真菌。结果在着色芽生菌病高发区山东章丘,354只野生蟾蜍中有151只分离出着色真菌,占42.7%,共计分离出着色真菌11种,162株,其中疣状瓶霉49株,外瓶霉33株,瓶霉属19株,甄氏外瓶霉19株,卡氏枝孢12株,可疑卡氏枝孢3株,喙枝孢7株,裴氏着色霉5株,毛样枝孢4株,棘状外瓶霉3株,枝孢属3株,小尾枝孢1株,未鉴定4株。而在无病区山东莱阳,45只蟾蜍中仅有4只分离出非致病性着色真菌,占8.9%。结论着色芽生菌病流行区野生蟾蜍真菌的分离阳性率显著高于非流行区,且分离出多种致病性着色真菌,提示野生蟾蜍带菌与着色芽生菌病的流行相关。
Objective To isolate pathogenic colored fungi from wild toad. Methods Tomato wild toad was isolated from high incidence area and non-infected area of colored blastomycosis, and the colored fungi were isolated from viscera. Results 151 pigmented blast fungi were isolated from 354 wild toadstools, accounting for 42.7% of the total. Among them, 11 pigmented fungi were isolated from 162 species, of which 49 were Verrucosa virens 33 strains of molds, 19 strains of vial molds, 19 strains of cisterns of Zhen-zhen, 12 strains of Corynebacterium cardum, 3 strains of susceptive Corynebacterium parvum, 7 strains of Corynebacterium parvum, 5 strains of Pseudomonas solani, 4 strains, 3 spines of Echinococcus, 3 strains of Cladosporium and 1 strain of Cladosporium spp., And 4 strains were unidentified. In the non-endemic area of Laiyang, Shandong, only 4 out of 45 toads isolated non-pathogenic colored fungi, accounting for 8.9%. Conclusion The positive rate of wild toadstool isolated from colored blastomycosis is significantly higher than that of non-endemic area, and a variety of pathogenic colored fungi are isolated, suggesting that the infection of wild toad is related to the prevalence of colored blastomycosis.