头颈鳞癌患者(HNSCC)存在细胞介导的免疫功能缺陷。已有实验表明HNSCC患者局部引流淋巴结的免疫能力也下降。对HNSCC患者颈部不同部位淋巴结中淋巴细胞的功能活性进行测定并作比较研究。所有淋巴结标本均取自根治性颈廓清术患者,分为靠近肿瘤淋巴结(NLNs)与远离肿瘤淋巴结(FLNs)组,分别分离出其中的淋巴细胞。实验分三步。首先比较NLNs中的淋巴细胞与FLNs中淋巴细胞的细胞毒作用。将2组淋巴细胞分别加入白细胞介素Ⅱ(IL-2)孵育4天,~(51)Cr释放测定技术检测其对肿瘤靶细胞系CAL-27的细胞毒作用。 Head and neck squamous cell carcinoma (HNSCC) cell-mediated immune dysfunction. Experiments have shown that HNSCC patients with locally draining lymph nodes also decreased immune capacity. The functional activity of lymphocytes in different parts of the neck of patients with HNSCC was measured and compared. All lymph node specimens were obtained from radical nephrectomy patients, and were divided into two groups: lymphocytes near the tumor nodes (NLNs) and distant from the tumor nodes (FLNs). The experiment is divided into three steps. First, the cytotoxic effect of lymphocytes in NLNs and lymphocytes in FLNs was compared. The two groups of lymphocytes were incubated with interleukin-2 (IL-2) for 4 days, and cytotoxicity against tumor cell line CAL-27 was detected by ~ (51) Cr release assay.