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逆境胁迫可引起植物体内H2O2升高,研究发现5mmol/L的H2O2外源抑制剂二甲基硫脲(Dimethylthiourea,DMTU)可促进瓶插月季提早开放并显著增大了花朵直径。为明确DMTU如何通过影响瓶插月季花瓣生理变化进而提高其观赏价值的机理,对5mmol/L DMTU处理下月季切花萨曼莎(Smantha)不同时间花瓣中抗氧化酶活性、蔗糖含量和蔗糖代谢酶活性进行了测定。结果表明,在瓶插早期DMTU处理显著降低了抗坏血酸过氧化物酶(APX)和过氧化物酶(POD)活性,在后期显著降低了过氧化氢酶(CAT)活性,提高了谷胱甘肽还原酶(GR)活性,推测APX和POD在瓶插早期起主要清除H2O2的作用,CAT和GR在后期起主要作用;DMTU显著提高了瓶插后期花瓣中的蔗糖含量以及早期蔗糖合成酶(SS)和磷酸蔗糖合成酶(SPS)的活性,推测DMTU可能通过维持瓶插月季花瓣的渗透压,维护花瓣内生理活动的正常进行,进而提高其观赏价值。
Under the stress of stress, H2O2 could be increased in plants. It was found that 5 mmol / L Dimethylthiourea (DMTU), an exogenous H2O2 inhibitor, could promote early opening of bottle rose and significantly increase flower diameter. In order to clarify how DMTU affects the petal physiological changes of rose plum and thus enhance its ornamental value, the antioxidant enzymes activity, sucrose content and sucrose metabolism enzyme (SOD) activity of petals of Smantha rose under 5 mmol / L DMTU treatment. Activity was measured. The results showed that DMTU significantly reduced the activity of ascorbate peroxidase (APX) and peroxidase (POD) in the early vial insertion stage and significantly reduced the activity of catalase (CAT) in the late stage and increased the activity of glutathione Reductase (GR). It is speculated that APX and POD play a major role in removing H2O2 from the early stage of vase plugging. CAT and GR play a major role in the later stage. DMTU significantly increased the content of sucrose in early petals and early sucrose synthase (SS ) And sucrose phosphate synthase (SPS) activity. It is speculated that DMTU may maintain the osmotic pressure of rose petals and maintain the normal physiological activities in petals, thus improving its ornamental value.