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目的应用PCR技术对奉贤地区部分公共场所空调冷却塔水及其他环境(淋浴设施等)进行军团菌污染状况调查。方法于2010-05和2010-10,分别采集中央空调冷却塔水样品及环境样品用聚合酶链(PCR)对其进行嗜肺军团菌检测,同时用常规法进行分型鉴定。结果采集的125份样品中,PCR法检出嗜肺军团菌45份,阳性检出率为36%,水样检出率远高于环境(分别为67.19%和3.28%)。在检出的59株军团菌中共鉴定出6种不同菌型,其中LP1为重点菌型,占50.85%。且在同一样品中检出不同菌型有2株以上的占35.29%。结论奉贤地区中央空调冷却塔及环境中存在军团菌污染。
Objective To investigate the status of Legionella contamination of air-conditioning cooling tower water and other environment (shower facilities) in some public places in Fengxian by PCR technique. Methods In 2010-05 and 2010-10, samples of water samples from cooling towers of central air-conditioners and environmental samples were respectively collected for the detection of Legionella pneumophila using polymerase chain reaction (PCR), and routine typing was also performed. Results Among the 125 samples collected, 45 Legionella pneumophila samples were detected by PCR. The positive detection rate was 36%. The detection rate of water samples was much higher than that of the environment (67.19% and 3.28% respectively). Of the 59 Legionella isolates detected, 6 different bacterial strains were identified, of which LP1 was the dominant bacterial species, accounting for 50.85%. In the same sample, more than 2 strains were detected, accounting for 35.29%. Conclusion There is Legionnaire contamination in central air conditioning cooling tower and environment in Fengxian district.