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利用铺有 Ｍａｔｒｉｇｅｌ的 Ｂｏｙｄｅｎ小室观察抗ＣＤ９、抗ＣＤ４２ａ、抗ＣＤ ６３、抗ＴＳＰ抗体对ＰＧ－ＣＬ３细胞侵袭作用的影响；采用流式细胞术观察２．５、１０、２０ｍｇ／Ｌ的川芎嗪、丹参酮ⅡＡ和５０、５００、１０００ Ｕ／Ｉ。的水蛙素和凝血酶对 ＰＧＣＬ３细胞膜表面 ＣＤ９、ＣＤ４２ａ、ＣＤ６３、ＴＳＰ表达的影响，观察川芎嗪、丹参酮ⅡＡ、水蛭素和凝血酶对人肺巨细胞癌ＰＧＣＬ３细胞表面血小板免疫相关抗原ＣＤ９、ＣＤ４２ａ、ＣＤ６３和ＴＳＰ表达的影响，部分阐述各种药物对肿瘤粘附和侵袭影响的不同作用机制。结果抗ＣＤ９抗体明显增加 ＰＧＣＬ３细胞的侵袭，而抗ＣＤ４２ａ、抗ＣＤ６３、抗ＴＳＰ抗体不同程度减少ＰＧＣＬ３细胞的侵袭。川芎嗪可使ＣＤ９＋、ＣＤ４２ａ＋、ＴＳＰ＋细胞的数目减少，ＰＧＣＬ３细胞表面ＣＤ９的表达减少；丹参酮ⅡＡ使ＣＤ９的表达有所增加，ＣＤ６３和 ＣＤ４２ａ的表达有所下降，ＣＤ４２ａ＋、ＴＳＰ＋的细胞数目下降；水蛭素使ＣＤ９、ＣＤ６３和ＣＤ４２ａ的表达有所减弱，ＣＤ４２ａ＋、ＣＤ６３＋细胞的比例下降；凝血酶使ＣＤ９和ＣＤ４２ａ的表达有所减弱，并使ＣＤ４２ａ＋的细胞数目减少。提示川芎嗪、丹参酮ⅡＡ、水蛭?
The effect of anti-CD9, anti-CD42a, anti-CD63, and anti-TSP antibodies on the invasion of PG-CL3 cells was observed using a Boyden chamber equipped with Matrigel; the tetramethylpyrazine at 2.5, 10, and 20 mg/L was observed by flow cytometry. Tanshinone IIA and 50, 500, 1000 U/I. The effects of ceruletin and thrombin on the expression of CD9, CD42a, CD63 and TSP on the surface of PGCL3 cell membrane. To observe the effects of tetramethylpyrazine, tanshinone IIA, hirudin and thrombin on platelet immune-associated antigen CD9 and CD42a on human lung giant cell carcinoma PGCL3 cells. The effects of the expression of CD63, TSP, and TSP on the different mechanisms of action of various drugs on tumor adhesion and invasion. Results Anti-CD9 antibody significantly increased the invasion of PGCL3 cells, while anti-CD42a, anti-CD63, and anti-TSP antibodies reduced the invasion of PGCL3 cells to different extents. Ligustrazine can reduce the number of CD9+, CD42a+ and TSP+ cells and decrease the expression of CD9 on PGCL3 cells. Tanshinone IIA increases the expression of CD9, CD63 and CD42a decrease, and the number of CD42a+ and TSP+ cells decreases. The expression of CD9, CD63 and CD42a was weakened, and the proportion of CD42a+ and CD63+ cells decreased. Thrombin weakened the expression of CD9 and CD42a and decreased the number of CD42a+ cells. Tips Ligustrazine, tanshinone IIA, leeches?