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目的：探讨激素和生长因子对体外培养人牙乳头细胞增殖的影响。方法：用ＭＴＴ法观察不同浓度的１，２５－二羟基维生素Ｄ３、胰岛素和表皮生长因子对离体培养的人牙乳头细胞生长的影响。结果：在１０－９～１０－７ｍｏｌ／Ｌ浓度范围时，１，２５－二羟基维生素Ｄ３对细胞增殖具有抑制作用，浓度越高，作用时间越长，抑制作用越强。胰岛素和生长因子对细胞增殖有一定促进作用，与浓度成正相关，分别在加药后第３天、４天作用最强。结论：在一定浓度和作用时间内，激素和生长因子对人牙乳头细胞的生长有调节作用
Objective: To investigate the effects of hormones and growth factors on the proliferation of human dental papilla cells cultured in vitro. Methods: The effects of different concentrations of 1,25-dihydroxyvitamin D3, insulin and epidermal growth factor on the growth of human dental papilla cells in vitro were observed by MTT assay. Results: In the concentration range of 10-9 ~ 10-7mol / L, 1,25-dihydroxyvitamin D3 had an inhibitory effect on cell proliferation. The higher the concentration, the longer the duration of action, the stronger the inhibitory effect. Insulin and growth factors have a certain role in promoting cell proliferation, which is positively correlated with the concentration. The effect is highest on the 3rd day and the 4th day after dosing. Conclusion: Hormones and growth factors have a regulatory effect on the growth of human dental papilla cells at a certain concentration and time